RBS

Part:BBa_K4951011:Design

Designed by: Julia Vu   Group: iGEM23_Stanford   (2023-10-08)


AmpR Toehold 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The preceding homology arms were designed to correspond with the biobrick prefix of BBa_I732097, an RFP ChlorR plasmid, for more streamlined insertion of the toehold into the plasmid backbone for in vivo toehold testing. The anterior homology arms were designed to correspond with the sfGFP gene to allow us to test the switches in a cell-free environment using the fluorescent gene as a reporter to quantitatively measure the expression level with and without the trigger sequence.


Source

This part was generated with NuPack software against the AmpR gene derived from an sfGFP plasmid retrieved from Dr. Phillip Kyriakakis, labeled pPK803.

References